Purpose: Wheat (Triticum aestivum L.), which is cultivated worldwide, is the epitome of how proteins and carbs combine to form human food. Drought stress is making it harder to produce wheat, a major abiotic constraint that reduces physiological and morphological performance. Methodology: This study investigates the effects of drought on the responses of two wheat varieties, Fakhre Bakhar (V1) and Dilkash (V2), to colchicine, three drought levels 100%, 70%, and 35% field capacity were examined both in the control and in 0.01 g colchicine treatment. Results: In addition to morphological and physiological parameters measurements at both 70% FC and 35% FC decreased the shoot fresh weight (35.7% and 22.1%, 58.2% and 41%), total chlorophyll (16.7% and 19.1%, and 38.1% and 35.1%), and No. of grains (24.1% and 20%, 41.3% and 31.4%) in both varieties. However, Colchicine treatment increased the shoot fresh weight (76.4% and 37%), total chlorophyll (37.3% and 31%), SOD (15% and 12.3%), and No. of grains (5.9% and 4.2%) while decreased MDA contents up to (12.9% and 12.7%). Drought consistently had a detrimental effect on most metrics; however, colchicine showed possibilities in mitigating the adverse effects of drought stress. Conclusion: Overall, colchicine showed promise as a potential strategy to improve drought tolerance in wheat.