Tetracycline (TC) is another antibiotic widely used in hospital and livestock farm treatments. Once used, these drugs are excreted from the body along with other waste products. Biological treatment is the preferred system for reducing the level of pollution in effluent before it is discharged into the environment. However, the quality of effluent treated by biological systems is still insufficient to remove low concentrations of antibiotic molecules, allowing them to persist and spread into the environment. This research aims to develop and evaluate the efficiency of an electrochemical wastewater treatment process. This study investigated the effect of Fe-coated anodes on 316L stainless steel (SS316L) surfaces to reduce TC concentration in synthetic wastewater. Two factors were studied: initial TC concentration and electrical potential on TC molecular removal efficiency were studied. The results showed that higher electrical potentials had higher TC molecular removal efficiency. At 24 volts (V) and an initial concentration of 30 mg/L, TC molecular removal reached 83%, followed by 18 V and 12 V with efficiencies of 67% and 50%, respectively. Furthermore, TC molecular removal efficiency was optimal at lower initial concentrations, achieving 70% removal at an initial concentration of 10 mg/L.