Climate change is increasing the frequency and severity of drought strain, which poses a first-rate task to grapevine production. This have a look at investigated the response of four grape rootstocks (Richter, Salt Creek, Freedom, and Dogridge) to water deficit prompted by way of polyethylene glycol (PEG). Two pressure induction techniques had been as compared: surprise remedy, related to direct exposure of cultures to growing PEG concentrations (0%, 2%, 4%, 6%, 8%, and 10%); and step-clever long-time period remedy, steadily increasing PEG concentrations (0%, 6%, 8%, and 10%) through the years. The essential findings were as follows: drought strain negatively impacted all rootstocks, leading to decreased morphological tendencies (shoot number, period, and root number), survival %, and biochemical parameters (chlorophyll a and b, carotenoids, stomata popularity, RWC content material). It additionally led to reduced nutrient accumulation (N, P, K, Mg, Ca) in leaves. However, all rootstocks exhibited increased Proline content and antioxidant enzyme hobby under all PEG concentrations. In phrases of rootstock-unique responses, Richter and Salt Creek showed the maximum sturdy performance, maintaining better shoot and root growth, nutrient content, and photosynthetic hobby compared to Freedom and Dogridge. On the alternative hand, Freedom and Dogridge exhibited extra sensitivity to drought stress, experiencing stronger discounts in boom, biochemical parameters, and nutrient accumulation. In conclusion Richter and Salt Creek rootstocks could be valuable equipment for reinforcing drought tolerance in grapevines. The two carried out PEG remedies provide valuable methods for screening and deciding on drought-tolerant grape rootstocks.